Proteases are extensively implicated in cancer development and progression. A new book in the Methods in Molecular Biology series aims to provide researchers with the latest methods for studying proteases. Proteases and Cancer Methods and Protocols contains chapters written by many of the leading researchers in the field. The book gets off to an excellent start with a very comprehensive and easy to follow review of the role of proteases in cancer. This is an impressive feat given that there are over 500 proteases in mammalian genomes. The following chapters include methods for genomic analysis of protease sequences and their expression levels, determination of substrate specificity and detection of their activity. The quality of the different chapters is generally high; although there is some variability depending on the particular authors. One criticism is that there is not enough coverage of the routine methodologies used in the field and their particular pros and cons. The book is competitively priced at £60 and would be a worthwhile buy for PhD students or postdocs starting out in this field, and institutional libraries.